Difference between revisions of "Part:BBa K2984054"
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<img src="https://2019.igem.org/wiki/images/d/df/T--Humboldt_Berlin--Konstruk_19.jpeg" alt="colonies_total" width="500"> | <img src="https://2019.igem.org/wiki/images/d/df/T--Humboldt_Berlin--Konstruk_19.jpeg" alt="colonies_total" width="500"> | ||
| − | <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | + | <figcaption>Fig.1 - Image of a successful transformation in <i>E. coli</i> after ligation of the construct. The construct can then be isolated from <i>E. coli</i> to be transformed in <i>C. reinhardtii</i>.</figcaption> |
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Latest revision as of 23:06, 19 October 2019
L1c-PsadIntron-bleRscp-ARS-YFP-SP20-Rbcs2
This vector is a part of the Chlamy-HUB-Collection. Level 1 construct containing secretion signal of arylsulfatase ARS fused to the flourescent protein mVenus and the SP20 glycomodule. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the PsaDintron for enhanced expression. The construct ends with the Rbcs2 terminator.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2051
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2051
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2051
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2051
Illegal NgoMIV site found at 1607
Illegal NgoMIV site found at 2528 - 1000COMPATIBLE WITH RFC[1000]
Characterization
