Difference between revisions of "Part:BBa K2984033"
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− | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c | + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c vector containing the arylsulfatase secretion peptide <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS1</a> and a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a> fluorescent marker, flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">RbcS2 terminator</a>. |
− | The construct is designed to test for the | + | The construct is designed to test for the efficiency of the secretion signal and the expression rates driven by the AR Promoter in <i>C. reinhardtii</i> by fluorescence intensity measurements. |
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Latest revision as of 13:32, 7 January 2020
L1c-AR-ARS-YFP-Rbcs2
This vector is a part of the Chlamy-HUB-Collection. L1c vector containing the arylsulfatase secretion peptide ARS1 and a mVenus fluorescent marker, flanked by AR promoter and RbcS2 terminator. The construct is designed to test for the efficiency of the secretion signal and the expression rates driven by the AR Promoter in C. reinhardtii by fluorescence intensity measurements.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 953
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 268
Illegal PstI site found at 953 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 953
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 953
Illegal NgoMIV site found at 1430 - 1000COMPATIBLE WITH RFC[1000]