Difference between revisions of "Part:BBa K2984029"

Latest revision as of 22:25, 19 October 2019

L1c-PsaD-GLE-PETase-YFP-SP20-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the GLE secretion signal, the PETase enzyme, a YFP mVenus marker, the SP20 glycomodule for enhanced secretion and the Rbcs2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1775
Illegal PstI site found at 1989
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1314
Illegal PstI site found at 1775
Illegal PstI site found at 1989
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 1038
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1775
Illegal PstI site found at 1989
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1775
Illegal PstI site found at 1989
Illegal NgoMIV site found at 2466
1000
COMPATIBLE WITH RFC[1000]

Characterization

colonies_total — Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii

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 <html>
-This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984001">GLE</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20</a> glycomodule for enhanced secretion and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a> (BBa_K29840). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984001">GLE</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984016">SP20</a> glycomodule for enhanced secretion and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part can be used to express and secrete the PETase enzyme in <i>C. reinhardtii</i>. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.
 </html>
@@ Line 14: / Line 14: @@
 <html>
 <figure>
-<img src="https://2019.igem.org/File:T--Humboldt_Berlin--Konstrukt_3.jpeg" alt="colonies_total" width="500">
+<img src="https://2019.igem.org/wiki/images/2/27/T--Humboldt_Berlin--Konstrukt_3.jpeg" alt="colonies_total" width="500">
 <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
 </figure>