Difference between revisions of "Part:BBa K2984028"

Latest revision as of 22:24, 19 October 2019

L1c-PsaD-ARS-PETase-YFP-SP20-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the ARS secretion signal, the PETase enzyme, a YFP mVenus marker, the SP20 glycomodule for enhanced secretion and the RbcS2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1769
Illegal PstI site found at 1983
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1308
Illegal PstI site found at 1769
Illegal PstI site found at 1983
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 1032
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1769
Illegal PstI site found at 1983
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1769
Illegal PstI site found at 1983
Illegal NgoMIV site found at 2460
1000
COMPATIBLE WITH RFC[1000]

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-This part is a part of the Chlamy-HUB-Collection. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20 glycomodule</a> for enhanced secretion and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">RbcS2 terminator</a>. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20 glycomodule</a> for enhanced secretion and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">RbcS2 terminator</a>. The part can be used to express and secrete the PETase enzyme in <i>C. reinhardtii</i>. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.
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