Difference between revisions of "Part:BBa K2984027"
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| − | L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements. | + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements. |
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K2984027 SequenceAndFeatures</partinfo> | ||
==Characterization== | ==Characterization== | ||
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<figure> | <figure> | ||
| − | <img src="igem" alt="colonies_total" width="500"> | + | <img src="https://2019.igem.org/wiki/images/d/d6/T--Humboldt_Berlin--Konstrukt_7.jpg" alt="colonies_total" width="500"> |
<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | ||
</figure> | </figure> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 08:58, 17 October 2019
L1c-Psad-ARS-YFP-Rbcs2
This vector is a part of the Chlamy-HUB-Collection. L1c plasmid containg arylsulfatase secretion signal ARS and mVenus fluorescent marker which are flanked by PsaD promoter and Rbcs2 Terminator. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1110
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1110
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1110
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1110
Illegal NgoMIV site found at 1587 - 1000COMPATIBLE WITH RFC[1000]
Characterization
