Difference between revisions of "Part:BBa K3226019:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | When PCR was performed, we were careful to use primers that insert a 6 × his tag between the start codon of RecA and the subsequent sequence. | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Dea Slade1,and Miroslav Radman,Oxidative Stress Resistance in Deinococcus radiodurans, Microbiol Mol Biol Rev. 2011 Mar; 75(1): 133–191. | ||
Latest revision as of 15:23, 17 October 2019
RecA+his
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 242
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 916
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When PCR was performed, we were careful to use primers that insert a 6 × his tag between the start codon of RecA and the subsequent sequence.
Source
Deinococcus Radioduranse
References
Dea Slade1,and Miroslav Radman,Oxidative Stress Resistance in Deinococcus radiodurans, Microbiol Mol Biol Rev. 2011 Mar; 75(1): 133–191.