Difference between revisions of "Part:BBa K105001"

 
(→‎Improvement)
 
(9 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K105001 short</partinfo>
 
<partinfo>BBa_K105001 short</partinfo>
Line 7: Line 6:
 
In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.   
 
In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.   
  
 +
===Improvement===
 +
2016 SZU-China has improved this parts and give it a new name ([https://parts.igem.org/Part:BBa_K1884002 BBa_K1884002])
 +
 +
<br\n>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 21:59, 19 October 2016

VP16 - eucaryotic activation domain

Originally VP16 is a protein from the herpes simplex virus. It activates the transcription of the late genes of this virus by the RNA-polymerase II of the eucaryotic host.

In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.

Improvement

2016 SZU-China has improved this parts and give it a new name (BBa_K1884002)

<br\n> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]