Difference between revisions of "Part:BBa K2960007:Design"

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===References===
 
===References===
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Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187

Latest revision as of 01:15, 21 October 2019


The mlrB serine protease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1124


Design Notes

The mlrB gene sequence has been codon-optimized for expression in E. coli.

The construct was designed to be RFC[10] compatible.

Source

Sphingopyxis sp. genomic sequence. European Nucleotide Archive. (2015 April 16). Received from https://www.ebi.ac.uk/ena/data/view/KR150744

References

Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187