Difference between revisions of "Part:BBa K143065:Design"
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<partinfo>BBa_K143065 short</partinfo> | <partinfo>BBa_K143065 short</partinfo> | ||
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<partinfo>BBa_K143065 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143065 SequenceAndFeatures</partinfo> | ||
+ | ===Design Notes=== | ||
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Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor. | Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor. | ||
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===Source=== | ===Source=== | ||
Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry | Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry | ||
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Latest revision as of 16:38, 9 October 2008
Spectinomycin Resistance Protein (Aad9) - Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 488
Design Notes
Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.
Source
Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry