Difference between revisions of "Part:BBa K2919000:Experience"
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This part was used in an attempt to make a toxin-antitoxin saftey system that would limit horizontal gene transfer. The part was used with a cumate inducible, CuO, promoter to prevent the toxin to kill the cell immediately. When induced with sufficient cumate the toxin worked to prevent cell growth in E. coli. | This part was used in an attempt to make a toxin-antitoxin saftey system that would limit horizontal gene transfer. The part was used with a cumate inducible, CuO, promoter to prevent the toxin to kill the cell immediately. When induced with sufficient cumate the toxin worked to prevent cell growth in E. coli. | ||
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| + | [[File:Cumate_ghoT_growthcurve.png]] | ||
| + | This graph show that the toxin, ghoT, works by showing that the E. coli with the toxin insert when induced by cumate had lower OD600s than the E. coli without the toxin insert. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 16:36, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2919000
This part was used in an attempt to make a toxin-antitoxin saftey system that would limit horizontal gene transfer. The part was used with a cumate inducible, CuO, promoter to prevent the toxin to kill the cell immediately. When induced with sufficient cumate the toxin worked to prevent cell growth in E. coli.
This graph show that the toxin, ghoT, works by showing that the E. coli with the toxin insert when induced by cumate had lower OD600s than the E. coli without the toxin insert.
User Reviews
UNIQ128139e49232b44c-partinfo-00000000-QINU UNIQ128139e49232b44c-partinfo-00000001-QINU