Difference between revisions of "Part:BBa K103004"
 
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<partinfo>BBa_K103004 short</partinfo>
 
<partinfo>BBa_K103004 short</partinfo>
  
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Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Bardzo chętnie agreguje (nawet podłączone do rozpuszczalnego białka), chyba że może związać B domain of Staphylococcal protein A (BBa_K103003).  
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===Usage and Biology===
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Artificially created strong interactor of B domain of Staphylococcal protein A ([[Part:BBa_K103003 | BBa_K103003]]). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A [http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum].
  
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*Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
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*We have used Z<sub>SPA-1</sub> protein fusions with [[Part:BBa_K103019 | BBa_K103019]] or [[Part:BBa_K103020 | BBa_K103020]] as a prey in our 'hunter and prey' selection system
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*This part uses our NdeI/SacI/BamHI fusion cloning substandard
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|[[Image:Z_spa.jpg]]
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Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K103004 parameters</partinfo>
 
<partinfo>BBa_K103004 parameters</partinfo>
 
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Latest revision as of 23:01, 28 October 2008

protein ZSPA-1

Artificially created strong interactor of B domain of Staphylococcal protein A ( BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A [http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum].

  • ZSPA-1-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
  • We have used ZSPA-1 protein fusions with BBa_K103019 or BBa_K103020 as a prey in our 'hunter and prey' selection system
  • This part uses our NdeI/SacI/BamHI fusion cloning substandard
Z spa.jpg

Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]