Difference between revisions of "Part:BBa K3173011"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K3173011 short</partinfo>
 
<partinfo>BBa_K3173011 short</partinfo>
  
−
TEV Protease with S1/3 + S2 sites for genetic knockout or tagging
+
TEV Protease with S1/3 + S2 homology sites for genetic knockout or tagging.
 +
Design homology primers (we recommend at least 40-60 bp homology to your genetic region) with S sites and run overhang PCR.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:26, 21 October 2019


TEV Protease - genetic tag or knockout

TEV Protease with S1/3 + S2 homology sites for genetic knockout or tagging. Design homology primers (we recommend at least 40-60 bp homology to your genetic region) with S sites and run overhang PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 2658
    Illegal PstI site found at 7
    Illegal PstI site found at 1193
    Illegal PstI site found at 1708
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 2658
    Illegal PstI site found at 7
    Illegal PstI site found at 1193
    Illegal PstI site found at 1708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 2658
    Illegal BglII site found at 983
    Illegal XhoI site found at 2402
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 2658
    Illegal PstI site found at 7
    Illegal PstI site found at 1193
    Illegal PstI site found at 1708
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 2658
    Illegal PstI site found at 7
    Illegal PstI site found at 1193
    Illegal PstI site found at 1708
  • 1000
    COMPATIBLE WITH RFC[1000]