Difference between revisions of "Part:BBa K3173010"
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<partinfo>BBa_K3173010 short</partinfo> | <partinfo>BBa_K3173010 short</partinfo> | ||
| − | STE2-with v2 Tail, efficient TCS and NLS-EGFP | + | STE2-with v2 Tail, efficient TCS and NLS-EGFP. |
| + | After addition of alpha mating factor TCS is accessible for Protease cleavage. | ||
| + | Use e.g. BBa_K3173011 to tag alpha arrestin regions in the yeast genome with TEV protease or subclone BBa_K3173012 as an orthognal part to introduce cleavage function for Tango like assay into yeast cells. | ||
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Latest revision as of 14:24, 21 October 2019
STE2-Tail-EGFP
STE2-with v2 Tail, efficient TCS and NLS-EGFP. After addition of alpha mating factor TCS is accessible for Protease cleavage. Use e.g. BBa_K3173011 to tag alpha arrestin regions in the yeast genome with TEV protease or subclone BBa_K3173012 as an orthognal part to introduce cleavage function for Tango like assay into yeast cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1442
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 709