Difference between revisions of "Part:BBa K103016"

Latest revision as of 00:48, 28 October 2008

OmpA_linker_omega_linker

It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit omega ( BBa_K103012)
Fusion partners are connected via Gly-Ser-Gly linker
It's base for creation of hunter fusions in 'hunter and prey' selection system
Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant
This part uses our NdeI/SacI/BamHI fusion cloning substandard

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_K103016 short</partinfo>
+{|
+|
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+*It's fusion of E. coli outer membrane protein (OmpA) domain ([[Part:BBa_K103006 | BBa_K103006]]) with beta-lactamase subunit omega ([[Part:BBa_K103012 | BBa_K103012]])
+*Fusion partners are connected via Gly-Ser-Gly linker
+*It's base for creation of hunter fusions in 'hunter and prey' selection system
+*Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
+*Interaction between hunter and prey (fused with [[Part:BBa_K103019 | BBa_K103019]]) makes cells ampicillin resistant
+*This part uses our NdeI/SacI/BamHI fusion cloning substandard
+| [[Image:Ompalinkomega.jpg|260px]]
+This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
+|}
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