Difference between revisions of "Part:BBa K3081104:Design"
 
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===Design Notes===
 
===Design Notes===
−
No
+
It already contains a terminator for DNA polymerase. But when you use some specific expression systems like T7, an extra corresponding terminator is needed.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 17: Line 15:
 
Previous publication:
 
Previous publication:
 
Qi L S , Larson M H , Gilbert L A , et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell, 2013, 152(5):1173-1183.v
 
Qi L S , Larson M H , Gilbert L A , et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell, 2013, 152(5):1173-1183.v
 +
Larson, M. H. , Gilbert, L. A. , Wang, X. , Lim, W. A. , Weissman, J. S. , & Qi, L. S. . (2013). Crispr interference (crispri) for sequence-specific control of gene expression. Nature Protocols, 8(11), 2180-2196.

Latest revision as of 14:20, 20 October 2019


scaffold of sgRNA in CRISPR/Cas system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

It already contains a terminator for DNA polymerase. But when you use some specific expression systems like T7, an extra corresponding terminator is needed.

Source

E.coli

References

Previous publication: Qi L S , Larson M H , Gilbert L A , et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell, 2013, 152(5):1173-1183.v Larson, M. H. , Gilbert, L. A. , Wang, X. , Lim, W. A. , Weissman, J. S. , & Qi, L. S. . (2013). Crispr interference (crispri) for sequence-specific control of gene expression. Nature Protocols, 8(11), 2180-2196.