Difference between revisions of "Part:BBa K143056"
m |
|||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K143056 short</partinfo> | <partinfo>BBa_K143056 short</partinfo> | ||
+ | |||
Inducible promoter xyl(<bbpart>BBa_K143014</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''. | Inducible promoter xyl(<bbpart>BBa_K143014</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''. | ||
+ | |||
Pxyl-gsiB can be used to take an input of xylose and give a '''Ribosomes per second''' (RiPS) output generator. | Pxyl-gsiB can be used to take an input of xylose and give a '''Ribosomes per second''' (RiPS) output generator. | ||
+ | |||
Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator '''XylR''', (<bbpart>BBa_K143036</bbpart>). This means that XylR must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by ''B. subtilis'' but should be upregulated to increase efficiency. | Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator '''XylR''', (<bbpart>BBa_K143036</bbpart>). This means that XylR must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by ''B. subtilis'' but should be upregulated to increase efficiency. | ||
+ | |||
'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.''' | '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.''' | ||
Line 16: | Line 19: | ||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><big>'''Sequence and Features'''</big></span> |
+ | |||
+ | |||
<partinfo>BBa_K143056 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143056 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===References=== | ||
+ | <biblio> | ||
+ | #1 https://parts.igem.org/Assembly:RBS-CDS_issues | ||
+ | </biblio> | ||
Latest revision as of 17:07, 8 October 2008
Promoter xyl and RBS gsiB for B. subtilis
Inducible promoter xyl(BBa_K143014) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.
Pxyl-gsiB can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.
Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, (BBa_K143036). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.
To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
</biblio>