Difference between revisions of "Part:BBa K143055"
m |
|||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K143055 short</partinfo> | <partinfo>BBa_K143055 short</partinfo> | ||
+ | |||
Inducible promoter hyper-spank(<bbpart>BBa_K143015</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. | Inducible promoter hyper-spank(<bbpart>BBa_K143015</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. | ||
+ | |||
Phyperspank-spoVG can be used to take an input of IPTG and give a '''Ribosomes per second''' (RiPS) output generator. | Phyperspank-spoVG can be used to take an input of IPTG and give a '''Ribosomes per second''' (RiPS) output generator. | ||
+ | |||
IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K143033</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank as an inducible promoter. | IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K143033</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank as an inducible promoter. | ||
+ | |||
'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.''' | '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.''' | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 15: | Line 19: | ||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><big>'''Sequence and Features'''</big></span> |
+ | |||
+ | |||
<partinfo>BBa_K143055 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143055 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | ===References=== | ||
+ | <biblio> | ||
+ | #1 https://parts.igem.org/Assembly:RBS-CDS_issues | ||
+ | </biblio> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Latest revision as of 17:06, 8 October 2008
Promoter hyper-spank and RBS spoVG for B. subtilis
Inducible promoter hyper-spank(BBa_K143015) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.
Phyperspank-spoVG can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.
IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (BBa_K143033). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.
To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
</biblio>