Difference between revisions of "Part:BBa K3117005"
 
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<partinfo>BBa_K3117005 short</partinfo>
 
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The part BBa_K3117005 is a 6xHis tag which can be used for protein purification and for the evidence of marked proteins. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns.  
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This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection (Booth et al. 2018).
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3117005 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3117005 parameters</partinfo>
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Latest revision as of 23:07, 21 October 2019


6xHis-Tag

This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection (Booth et al. 2018).