Difference between revisions of "Part:BBa K3110044"

 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3110044 short</partinfo>
 
<partinfo>BBa_K3110044 short</partinfo>
  
There is constitutive Promoter (J23117) followed by Medium RBS (B0034) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).
+
There is constitutive Promoter (J23117) followed by Medium RBS (B0032) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).
  
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
Line 17: Line 15:
 
<partinfo>BBa_K3110044 parameters</partinfo>
 
<partinfo>BBa_K3110044 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<h1>Characterization </h1>
 +
Due to shortage of time we could not characterize this construct.

Latest revision as of 15:59, 21 October 2019


J23117 Medium RBS sfGFP

There is constitutive Promoter (J23117) followed by Medium RBS (B0032) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 67


Characterization

Due to shortage of time we could not characterize this construct.