Latest revision as of 15:59, 21 October 2019

J23117 Medium RBS sfGFP

There is constitutive Promoter (J23117) followed by Medium RBS (B0032) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 67

Characterization

Due to shortage of time we could not characterize this construct.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3110044 short</partinfo>
-There is constitutive Promoter (J23117) followed by Medium RBS (B0034) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).
+There is constitutive Promoter (J23117) followed by Medium RBS (B0032) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
@@ Line 17: / Line 15: @@
 <partinfo>BBa_K3110044 parameters</partinfo>
 <!-- -->
+<h1>Characterization </h1>
+Due to shortage of time we could not characterize this construct.

Difference between revisions of "Part:BBa K3110044"

Latest revision as of 15:59, 21 October 2019

Characterization