Difference between revisions of "Part:BBa K3081009:Design"

Latest revision as of 09:28, 19 October 2019

pBAD-dCas9-J23119-R1+(18bp)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
Illegal NheI site found at 5459
Illegal NheI site found at 5482
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1470
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

Design Notes

no

Source

E.coli

References

Qi, L. S. , Larson, M. H. , Gilbert, L. A. , Doudna, J. A. , & Lim, W. A. . (2013). Repurposing crispr as an rna-guided platform for sequence-specific control of gene expression. Cell, 152(5), 1173-1183.

Revision as of 19:00, 16 October 2019 (view source) Registry (Talk \| contribs)		Latest revision as of 09:28, 19 October 2019 (view source) Lelezhang (Talk \| contribs)
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	===References===		===References===
		+	Qi, L. S. , Larson, M. H. , Gilbert, L. A. , Doudna, J. A. , & Lim, W. A. . (2013). Repurposing crispr as an rna-guided platform for sequence-specific control of gene expression. Cell, 152(5), 1173-1183.