Difference between revisions of "Part:BBa K2999010"
 
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<partinfo>BBa_K2999010 short</partinfo>
 
<partinfo>BBa_K2999010 short</partinfo>
  
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PrtN, a lysozyme activating protein of Pseudomonas aeruginosa PAO1, was linked to PA2069, a promoter regulated by HSL signal molecule of PAO1 quorum sensing system, as a delayed expression system to cleave PAO1.
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PrtN, a lysozyme activating protein of ''Pseudomonas aeruginosa'' PAO1, was linked to PA2069, a promoter regulated by HSL signal molecule of PAO1 quorum sensing system, as a delayed expression system to cleave PAO1.
  
  
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[[File:T--YAU-China--iGEM2019-BBa -8.png|500px|thumb|none|Figure 1. The Yau China team cultured the experimental strain and the control group in LB liquid medium respectively, and detected OD600 every 6 hours with enzyme labeling instrument. It can be seen that the experimental strain began to decline obviously after growing to the stable stage.]]
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[[File:T--YAU-China--iGEM2019-BBa -8.png|500px|thumb|none|Figure 1. The YAU-China team cultured the experimental strain and the control group in LB liquid medium respectively, and detected OD600 every 6 hours with enzyme labeling instrument. It can be seen that the experimental strain began to decline obviously after growing to the stable stage.]]
  
 
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Latest revision as of 05:56, 18 October 2019


pPA2069+RBS+PrtN

PrtN, a lysozyme activating protein of Pseudomonas aeruginosa PAO1, was linked to PA2069, a promoter regulated by HSL signal molecule of PAO1 quorum sensing system, as a delayed expression system to cleave PAO1.


Usage and Biology

Figure 1. The YAU-China team cultured the experimental strain and the control group in LB liquid medium respectively, and detected OD600 every 6 hours with enzyme labeling instrument. It can be seen that the experimental strain began to decline obviously after growing to the stable stage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 365
    Illegal XhoI site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 271
    Illegal NgoMIV site found at 297
    Illegal NgoMIV site found at 482
    Illegal NgoMIV site found at 602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 989