Difference between revisions of "Part:BBa K3053069"

 
 
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This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed.
 
This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed.
  
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===Usage and Biology===
 
===Usage and Biology===
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<p>The part is designed in such a way that it is complementary to the RBS (BBa_K3503001). This way, when no ampicillin resistance gene is present (<i>bla</i>) it forms a double stem loop with the RBS and inhibits the expression of the Cro gene which induces the lysogenic cycle of the phage.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3053069 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3053069 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:38, 21 October 2019


Reverse Complement

This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed.

Usage and Biology

The part is designed in such a way that it is complementary to the RBS (BBa_K3503001). This way, when no ampicillin resistance gene is present (bla) it forms a double stem loop with the RBS and inhibits the expression of the Cro gene which induces the lysogenic cycle of the phage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]