Difference between revisions of "Part:BBa K3081119"

 
 
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<partinfo>BBa_K3081119 short</partinfo>
 
<partinfo>BBa_K3081119 short</partinfo>
  
In this part, sfGFP is fused to the C terminus of dCas9, and we use it to evaluate the expression of dCas9.  
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In this part, sfGFP is fused to the C terminus of dCas9, and we use it to evaluate the expression of dCas9. For more detailed information, see <partinfo>BBa_K3081002</partinfo>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:15, 19 October 2019


dCas9-linker-sfGFP

In this part, sfGFP is fused to the C terminus of dCas9, and we use it to evaluate the expression of dCas9. For more detailed information, see BBa_K3081002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4147