Difference between revisions of "Part:BBa K3171172:Design"

 
 
Line 17: Line 17:
  
 
===References===
 
===References===
 +
 +
Sarah E. Aiyar, Tamas Gaal, and Richard L. Gourse rRNA Promoter Activity in the Fast-Growing Bacterium Vibrio natriegens. J Bacteriol. 2002 Mar; 184(5): 1349–1358. doi: 10.1128/JB.184.5.1349-1358.2002

Latest revision as of 11:42, 21 October 2019


Vibrio natriegens Native P1 promotor with mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 305
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal SpeI site found at 306
    Illegal PstI site found at 320
    Illegal NotI site found at 13
    Illegal NotI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 306
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 7
    Illegal XbaI site found at 22
    Illegal SpeI site found at 306
    Illegal PstI site found at 320
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

P1+mCherry fusion


Source

mCherry was obtained from the registry BBa_J06602. Native P1 promoter was obtained from IDT

References

Sarah E. Aiyar, Tamas Gaal, and Richard L. Gourse rRNA Promoter Activity in the Fast-Growing Bacterium Vibrio natriegens. J Bacteriol. 2002 Mar; 184(5): 1349–1358. doi: 10.1128/JB.184.5.1349-1358.2002