Difference between revisions of "Part:BBa K3081108"

Latest revision as of 19:18, 21 October 2019

a 20bp sequence targeting the R1 (bottom strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]

Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3081108 short</partinfo>
-a 20bp sequence targeting the R1+ DnaA box on E.coli oriC
+The 20 bp sgRNA targets to the R1+ DnaA box on <i>E.coli</i> genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
+<center>
+https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
+</center>
+<center>
+Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
+</center>
+Reference:
+[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
 <!-- Add more about the biology of this part here