Difference between revisions of "Part:BBa K3093001:Design"

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(Design Notes)
 
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===Design Notes===
 
===Design Notes===
we have remove the strong NagC binding site...
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The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site.
 
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===Source===
 
===Source===

Latest revision as of 01:07, 17 October 2019


chbB promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site.

Source

Escherichia coli str. K-12 substr.MG1655

References

[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.