Difference between revisions of "Part:BBa K3264023:Design"

 
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===Design Notes===
 
===Design Notes===
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an C-terminal domain based on the E. australis MiSp1 sequence
 
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(VTSG GYGYGTSAAA GAGVAAGSYA GAVNRLSSAE AASRVSSNIA AIASGGASAL PSVISNIYSG VVASGVSSNE ALIQALLELL SALVHVLSSA SIGNVSSVGV DSTLNVVQDS VGQYVG)
 
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Also, the linker between the CT and the Rep (repetitive region) is GNS
 
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The construct was cloned into a pT7 plasmid and transformed into BL21 (DE3) E. coli bonded to NT-2Rep-CT
 
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===Source===
 
===Source===
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de novo synthesize
 
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DNA from Araneus ventricosus
 
  
 
===References===
 
===References===

Latest revision as of 15:28, 16 October 2019


TALEsp-vioA-vioB-vioC-vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References