Difference between revisions of "Part:BBa K3007027"

Latest revision as of 14:38, 16 October 2019

Reverse version of BBa_K3007025

Hypothetical uricase regulator (HucR) from D. radiodurans is a bacterial transcriptional factor, which acts as a homo-dimer and binds to hucO in the absence of uric acid. When uric acid is present, HucR dissociates from hucO, allowing expression of a downstream gene. YgfU is a transporter for importing uric acid into cytoplasm in E. coli. In order to simplify the experiment design, QHFZ-China 2019 added the sequence coding YgfU after the CDS of HucR to transport and detect the exocellular uric acid.

It is a reverse version of BBa_K3007024, which is used to document a bigger composite part.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 309
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 309
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 309
Illegal BamHI site found at 1176
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 309
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 309
Illegal AgeI site found at 994
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3007027 short</partinfo>
-It is a reverse version of BBa_K3007025, which are used to document a bigger composite part.
+Hypothetical uricase regulator (HucR) from D. radiodurans is a bacterial transcriptional factor, which acts as a homo-dimer and binds to hucO in the absence of uric acid. When uric acid is present, HucR dissociates from hucO, allowing expression of a downstream gene. YgfU is a transporter for importing uric acid into cytoplasm in E. coli. In order to simplify the experiment design, QHFZ-China 2019 added the sequence coding YgfU after the CDS of HucR to transport and detect the exocellular uric acid.
+It is a reverse version of BBa_K3007024, which is used to document a bigger composite part.
 <!-- Add more about the biology of this part here