Difference between revisions of "Part:BBa K3286045"

 
 
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<partinfo>BBa_K3286045 short</partinfo>
 
<partinfo>BBa_K3286045 short</partinfo>
  
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Red fluorescent protein (RFP) and Green fluorescent protein (GFP) downstream of bacteriophage Lambda promoters, UTR's and operator regions OL and OR.
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A monomeric red fluorescent protein (mRFP) in antisense order.  
  
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This construct contains the regulatory regions and promoters of bacteriophage Lambda and has been used to asses a for the potential inhibition of bacteriophage LAmbda by CRISPRi.
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<ul>Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. https://doi.org/10.1073/pnas.082243699</ul>
  
 
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Latest revision as of 16:02, 20 October 2019


Monomeric red fluorescent protein (mRFP)

A monomeric red fluorescent protein (mRFP) in antisense order.


    Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. https://doi.org/10.1073/pnas.082243699

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]