Difference between revisions of "Part:BBa K2936012"

Latest revision as of 06:39, 19 October 2019

araBAD promoter

The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction. araC encodes the AraC protein, which regulates activity of both the PBAD and PC promoters.

Usage and Biology

Experiment

Figure 1 (a) the pBAD gene of E. coli does include the binding sites of araC plasmid(b)the pBAD gene of E. coli does not include the binding sites of araC plasmid, LacZ alpha gene encodes the β-galactosidase, which could react with ONPG to generate orange color.

Reference

[1]Vladislav V Verkhusha, Konstantin A Lukyanov, (2004) The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. [J]. Nature Biotechnology (2004), 289-296
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 120
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+===<h1>Experiment</h1>===
+<p>
+<html>
+  <img src="https://static.igem.org/mediawiki/parts/4/4f/T--ZJUT-China--igem-part12yanse.png" alt=""width="600px">
+</html>
+<br>
+Figure 1 (a) the pBAD gene of E. coli does include the binding sites of araC plasmid(b)the pBAD gene of E. coli does not include the binding sites of araC plasmid, LacZ alpha gene encodes the β-galactosidase, which could react with ONPG to generate orange color.
+</p>
+===Reference===
+[1]Vladislav V Verkhusha, Konstantin A Lukyanov,  (2004) The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. [J]. Nature Biotechnology (2004), 289-296<br>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>