Difference between revisions of "Part:BBa K3268000"

Latest revision as of 15:16, 16 October 2019

mOR103-15

This part codes for mouse olfactory receptor protein, which can bind with heptanal.
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004).
We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
2. Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 454
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 428
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 834

@@ Line 11: / Line 11: @@
 . The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
 <br>
-. Since the mOR103-15 coding sequence contains a BamHI cutting site, we have designed two PCR primers with HindIII and BglII sites respectively used for this amplification.
+. Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification.
+<html>
+<div>
+<img src="https://static.igem.org/mediawiki/parts/thumb/3/3e/T--NYMU-Taipei_J364007_with_mOR103-15_CDS.png/643px-T--NYMU-Taipei_J364007_with_mOR103-15_CDS.png" width="60%" height="60%">
+</div>
+</html>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===