Difference between revisions of "Part:BBa K3268000"
 
(5 intermediate revisions by the same user not shown)
Line 6: Line 6:
 
<br>
 
<br>
 
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004).  
 
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004).  
 +
<br>
 
We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
 
We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
 
<br>
 
<br>
 
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
 
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
 
<br>
 
<br>
−
2. The two PCR primers used for this amplification contain HindII and BamHI sites, respectively.
+
2. Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification.
−
 
+
<html>
 +
<div>
 +
<img src="https://static.igem.org/mediawiki/parts/thumb/3/3e/T--NYMU-Taipei_J364007_with_mOR103-15_CDS.png/643px-T--NYMU-Taipei_J364007_with_mOR103-15_CDS.png" width="60%" height="60%">
 +
</div>
 +
</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 15:16, 16 October 2019


mOR103-15

This part codes for mouse olfactory receptor protein, which can bind with heptanal.
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004).
We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
2. Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 834