Difference between revisions of "Part:BBa K3196028"

 
(→‎Functional parameters (Added by XJTU-China 2021))
 
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__NOTOC__
 
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{|width='80%'
 
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|width=35% valign='top'|
 
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K3196028 parameters</partinfo>
 
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<partinfo>BBa_K3196028 short</partinfo>
 
<partinfo>BBa_K3196028 short</partinfo>
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* tt
 
  
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promoter
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<h1>'''Characterization'''</h1>
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Pgas, a low-pH-inducible promoter, promoted gene expression efficiently at low pHs, such as pH 2.0.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3196028 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3196028 parameters</partinfo>
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<!-- -->
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===Functional parameters (Added by XJTU-China 2021)===
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As is shown in '''Fig.1''' (Yin et al., 2017), the strength of Pgas is mainly dependent on the pH, while limited influenced by the types of acids.
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[[File:XJTU-China-Pgas-ref.png|300px]]
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'''''Fig. 1''''' ''Factors affecting relative Pgas-inducing capacity. (A) Influence of acid type on Pgas-inducing capacity. (B) Influence of citrate concentration on Pgas-inducing capacity. (C) Fold changes in CAD expression at different pHs. Error bars represent the standard errors of the means of results for three biological replicates.''
  
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'''Secondary Structure'''
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The author gives an equation with linear regression analysis to describe the relationship between the relative expression level and pH:
  
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[[Image:Mfold-K3196028-1.png]]
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[[File:XJTU-China-Pgas-ref2.png|300px]]
  
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<hr>
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in which, E represents the relative expression level compared with expression at pH 7.0. The correlation coefficient factor (R2) is 0.9962.
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'''Measurement'''
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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Latest revision as of 08:23, 20 October 2021


Pgas

promoter

Characterization

Pgas, a low-pH-inducible promoter, promoted gene expression efficiently at low pHs, such as pH 2.0.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional parameters (Added by XJTU-China 2021)

As is shown in Fig.1 (Yin et al., 2017), the strength of Pgas is mainly dependent on the pH, while limited influenced by the types of acids.

XJTU-China-Pgas-ref.png

Fig. 1 Factors affecting relative Pgas-inducing capacity. (A) Influence of acid type on Pgas-inducing capacity. (B) Influence of citrate concentration on Pgas-inducing capacity. (C) Fold changes in CAD expression at different pHs. Error bars represent the standard errors of the means of results for three biological replicates.

The author gives an equation with linear regression analysis to describe the relationship between the relative expression level and pH:

XJTU-China-Pgas-ref2.png

in which, E represents the relative expression level compared with expression at pH 7.0. The correlation coefficient factor (R2) is 0.9962.