Difference between revisions of "Part:BBa K2984036"
 
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<partinfo>BBa_K2984036 short</partinfo>
 
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This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP mVenus marker (BBa_K2984024), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.  
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a> (BBa_K2984034), the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> marker, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the PETase enzyme in <i>C. reinhardtii</i>. The YFP-mVenus tag can be used to detect the expression of the PETase.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2984036 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984036 SequenceAndFeatures</partinfo>
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==Characterization==
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<figure>
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<img src="https://2019.igem.org/wiki/images/3/3d/T--Humboldt_Berlin--Konstrukt_6.jpeg" alt="colonies_total" width="500">
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<figcaption>Fig.1 - Image of a successful transformation in <i>E.coli</i> after ligation of the construct. The construct can then be isolated from <i>E.coli</i> to be transformed in <i>C. reinhardtii</i></figcaption>
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Latest revision as of 22:31, 19 October 2019


L1c-PsaD-PETase-YFP-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker (BBa_K2984034), the PETase enzyme, a YFP mVenus marker, and the Rbcs2 terminator. The part can be used to express the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1244
    Illegal PstI site found at 1915
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1915
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1915
    Illegal NgoMIV site found at 2392
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E.coli to be transformed in C. reinhardtii