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	<partinfo>BBa_K3196022 short</partinfo>		<partinfo>BBa_K3196022 short</partinfo>
−	protein	+	FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.
−	<!-- Add more about the biology of this part here	+	<h1>'''Characterization'''</h1>
−	===Usage and Biology===	+	This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.<sup>[1]</sup> It can help the peptide chain of VP be transferred out cells. [[File:T--HUST--China--2019-FLO10-αproVP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]]
		+
		+	<h1>'''Reference'''</h1>
		+	[1]Effect of signal peptides on the expression of laccase in Pichia pastoris  Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong  Acta Microbiologica Sinica 54(12) :1446 － 1452; 4 December 2014 ISSN 0001 － 6209; CN 11 － 1995 /Q
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Latest revision as of 10:39, 20 October 2019

AOX1-Kozak-FLO10 pro-VP-His tag-AOX1 Terminator

FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.^[1] It can help the peptide chain of VP be transferred out cells.

Reference

[1]Effect of signal peptides on the expression of laccase in Pichia pastoris Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong Acta Microbiologica Sinica 54(12) :1446 － 1452; 4 December 2014 ISSN 0001 － 6209; CN 11 － 1995 /Q

Sequence and Features