Difference between revisions of "Part:BBa K2982008"
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A reverse primer that is 31 base pairs long that can be used to amplify the GFP part BBa_E0040 by PCR. | A reverse primer that is 31 base pairs long that can be used to amplify the GFP part BBa_E0040 by PCR. | ||
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| + | It is used in conjunction with BBa_K2982006 or BBa_K2982006 in order to amplify a sequence that has an RBS with GFP (BBa_E0040). This is done for characterisation of BBa_J61124 by measuring its activity as an RBS. | ||
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| + | This primer contains an XhoI restriction site and the last 21 nucleotides of the GFP sequence. | ||
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Latest revision as of 14:36, 21 October 2019
Reverse primer for amplifying BBa_E0040
A reverse primer that is 31 base pairs long that can be used to amplify the GFP part BBa_E0040 by PCR.
It is used in conjunction with BBa_K2982006 or BBa_K2982006 in order to amplify a sequence that has an RBS with GFP (BBa_E0040). This is done for characterisation of BBa_J61124 by measuring its activity as an RBS.
This primer contains an XhoI restriction site and the last 21 nucleotides of the GFP sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 5
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]