Difference between revisions of "Part:BBa K2982006"
 
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<partinfo>BBa_K2982006 short</partinfo>
 
<partinfo>BBa_K2982006 short</partinfo>
  
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A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring OD.
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A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence.
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This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:36, 21 October 2019


Forward primer with BBa_B0034 for amplifying BBa_E0040

A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence.


This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]