Difference between revisions of "Part:BBa K2982006"
TitusWasHere (Talk | contribs) |
|||
| (3 intermediate revisions by 2 users not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2982006 short</partinfo> | <partinfo>BBa_K2982006 short</partinfo> | ||
| − | A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by PCR. It can be used to characterise the RBS activity easily by measuring | + | A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence. |
| + | |||
| + | |||
| + | This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 14:36, 21 October 2019
Forward primer with BBa_B0034 for amplifying BBa_E0040
A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence.
This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5
- 1000COMPATIBLE WITH RFC[1000]