Difference between revisions of "Part:BBa K2908675:Design"

 
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===Design Notes===
 
===Design Notes===
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he initiation codon ATG can only appear between miRNA sponse and EGFP.
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The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture.
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https://static.igem.org/mediawiki/parts/7/78/T--CSU_CHINA--ggmodule2.png
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===Source===
 
===Source===
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===References===
 
===References===
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Waclaw Szybalski”, Sun C. Kim”, Noaman Hasan” and Anna J. Podhajskab, Class IIS restriction enzymes, a review.1991, Gene, 100: 13-26.

Latest revision as of 21:52, 17 October 2019


s(ESR1)p-miR101spe-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture. T--CSU_CHINA--ggmodule2.png

Source

We designed it by ourselves and constructed this part by Golden gate.

References

Waclaw Szybalski”, Sun C. Kim”, Noaman Hasan” and Anna J. Podhajskab, Class IIS restriction enzymes, a review.1991, Gene, 100: 13-26.