Difference between revisions of "Part:BBa K2984033"

 
 
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<partinfo>BBa_K2984033 short</partinfo>
 
<partinfo>BBa_K2984033 short</partinfo>
  
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L1c plasmid containg arylsulfatase secretion signal and mVEnus fluorescent marker which are flanked by AR Promoter and Rbcs2 Terminator.
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The construct is designed to test for the efficency of the secretion Signal and the expression rates of the AR Promoter in C.reinhardtii with fluorescence intensity measurements.  
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c vector containing the arylsulfatase secretion peptide <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS1</a> and a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a> fluorescent marker, flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">RbcS2 terminator</a>.
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The construct is designed to test for the efficiency of the secretion signal and the expression rates driven by the AR Promoter in <i>C. reinhardtii</i> by fluorescence intensity measurements.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:32, 7 January 2020


L1c-AR-ARS-YFP-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. L1c vector containing the arylsulfatase secretion peptide ARS1 and a mVenus fluorescent marker, flanked by AR promoter and RbcS2 terminator. The construct is designed to test for the efficiency of the secretion signal and the expression rates driven by the AR Promoter in C. reinhardtii by fluorescence intensity measurements.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal PstI site found at 953
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
    Illegal NgoMIV site found at 1430
  • 1000
    COMPATIBLE WITH RFC[1000]