Difference between revisions of "Part:BBa K3102019:Design"

Latest revision as of 09:28, 16 October 2019

DLD-II operon

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4330
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We mutated a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme.

We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme.

Source

This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU

References

Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.

@@ Line 8: / Line 8: @@
 ===Design Notes===
-We mutated a Cysteine  into Thymine and a Guanine into Adenine at the 471bp and 476bp position (C471T & G476A), respectively, because of the PstI enzyme.
+We mutated a Cysteine  into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme.
-We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2842bp and 2845bp position (A2842G & C2845T), respectively, because of the EcoRI enzyme.
+We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme.
 ===Source===