Difference between revisions of "Part:BBa K3102035:Design"

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===Design Notes===
 
===Design Notes===
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We mutated in the MtrC sequence a Guanine into Cysteine and a Guanine into Adenine at the 1838bp and 1841bp position (G1838C & G1841A), respectively, because of the PstI enzyme.
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In the DLD-II operon, we mutated a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme.  
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We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019)
  
 
===Source===
 
===Source===

Latest revision as of 09:58, 16 October 2019


T7-RBS-DLDII Operon-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the DLD-II operon, we mutated a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme. We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019)

Source

This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.