Difference between revisions of "Part:BBa K3102045:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | We mutated the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1795bp and 1798bp position (G1795C & G1798A), respectively, because of the PstI enzyme (BBa_K3102020). | ||
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+ | The DLD-II operon sequence was mutated as followed : | ||
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+ | - a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme. | ||
+ | |||
+ | - a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019) | ||
===Source=== | ===Source=== | ||
− | DLD-II | + | DLD-II: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU |
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MtrC: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627 | MtrC: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627 | ||
Latest revision as of 09:57, 16 October 2019
Synthesis and externalization of lactate (DLD-II Operon, MtrC)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4365
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4949
Design Notes
We mutated the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1795bp and 1798bp position (G1795C & G1798A), respectively, because of the PstI enzyme (BBa_K3102020).
The DLD-II operon sequence was mutated as followed :
- a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme.
- a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019)
Source
DLD-II: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU
MtrC: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627
Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.