Difference between revisions of "Part:BBa K143062"
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− | LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>. | + | LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>). |
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− | LacI can be used in conjunction with the lac operon promoter (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''Polymerases per second''' (PoPS) output. | + | The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with ''B. subtilis''. LacI can be used in conjunction with the lac operon promoter (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''Polymerases per second''' (PoPS) output. |
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The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. | The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. | ||
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The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit. | The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit. | ||
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+ | Please note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree. | ||
Latest revision as of 02:48, 30 October 2008
LacI repressor protein - Terminator
LacI transcriptional repressor protein (BBa_K143033) coupled to the double terminator (BBa_B0015).
The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis. LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.
The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.
The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.
Please note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]