Difference between revisions of "Part:BBa K2926057"

 
 
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<partinfo>BBa_K2926057 short</partinfo>
 
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Mating factor alpha from S. cerevisiae was fused n-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the c-terminus of mCherry. To enable easy purificatio of the fusion protein a hexahistidine tag was fused to the c-terminus.
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Mating factor alpha from S. cerevisiae was fused N-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy purification of the fusion protein a hexahistidine tag was fused to the C-terminus.
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h1>Sequence and Features</h1></span>
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Sequence was validated by Sanger sequencing.
 
<partinfo>BBa_K2926057 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2926057 SequenceAndFeatures</partinfo>
  

Latest revision as of 22:58, 17 October 2019


Fusion protein of mating factor alpha, mCherry and pVIII with his-tag

Mating factor alpha from S. cerevisiae was fused N-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy purification of the fusion protein a hexahistidine tag was fused to the C-terminus.


Sequence and Features

Sequence was validated by Sanger sequencing.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]