Difference between revisions of "Part:BBa K3317001"

Latest revision as of 23:10, 21 October 2019

Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6 with the pduP signal peptide

Vanillate O-demethylase (LigM)(BBa_K3317000), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et. al 2017) This dimeric enzyme is tetrahydrofolate-dependent.

Wild type enzyme information: E. C. 2.1.1.341

Km 0.078

Kcat 7.1292

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 369
Illegal NgoMIV site found at 1150
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1372

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3317001 short</partinfo>
-Vanillate O-demethylase (LigM)(<partinfo>K3317000</partinfo>), was modified with a signal peptide at its C-terminal () to be introduced into bacterial microcompatments (BMC)
+Vanillate O-demethylase (LigM)(<partinfo>K3317000</partinfo>), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J <i> et. al</i> 2017)
+This dimeric enzyme is tetrahydrofolate-dependent.
+<html><img style="padding: 0 15px; height: 200px; weight: 200px float: right;" src="https://2019.igem.org/wiki/images/3/35/T--UANL--parts--ligps.png"></html>
+Wild type enzyme information:
+E. C. 2.1.1.341
+Km 0.078
+Kcat 7.1292
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