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Latest revision as of 21:53, 15 October 2019

Phosphite oxidoreductase (ptxD) B3-B4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 967
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon syntax fits C. reinhardtii. This was designed as a B3-B4 part according to the MoClo standard of Patron et al. (2015).


Source

Loera-Quezada, M. M., Leyva-González, M. A., Velázquez-Juárez, G., Sanchez-Calderón, L., Do Nascimento, M., López-Arredondo, D., & Herrera-Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant Biotechnol J, 14(10), 2066-2076.

References

Loera-Quezada, M. M., Leyva-Gonzalez, M. A., Lopez-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Sci, 231, 124-130. doi:10.1016/j.plantsci.2014.11.015. Loera-Quezada, M. M., Leyva-González, M. A., Velázquez-Juárez, G., Sanchez-Calderón, L., Do Nascimento, M., López-Arredondo, D., & Herrera-Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant Biotechnol J, 14(10), 2066-2076. Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19.