Difference between revisions of "Part:BBa K3102045:Design"

 
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===Design Notes===
 
===Design Notes===
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none
 
  
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We mutated the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1795bp and 1798bp position (G1795C & G1798A), respectively, because of the PstI enzyme (BBa_K3102020).
  
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The DLD-II operon sequence was mutated as followed :
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 +
- a Cysteine  into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme.
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 +
- a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019)
  
 
===Source===
 
===Source===
  
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none
+
DLD-II: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU
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MtrC: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627
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Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
  
 
===References===
 
===References===
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Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.

Latest revision as of 09:57, 16 October 2019


Synthesis and externalization of lactate (DLD-II Operon, MtrC)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4949


Design Notes

We mutated the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1795bp and 1798bp position (G1795C & G1798A), respectively, because of the PstI enzyme (BBa_K3102020).


The DLD-II operon sequence was mutated as followed :

- a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme.

- a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme. (BBa_K3102019)

Source

DLD-II: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU

MtrC: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.