Difference between revisions of "Part:BBa K3102044"
 
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This electron transfer improvement (ACS, ZWF, GND, PGL, Riboflavin, RibF) composite is composed of two main parts of sequences.
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__NOTOC__
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<partinfo>BBa_K3102044 short</partinfo>
  
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The first one is composed of acetyl-CoA synthetase (ACS): BBa_K3102031 to generate acetyl-coA by degrading acetate but also of G6P dehydrogenase (ZWF): BBa_K3102032, 6-phosphogluconate dehydrogenase (GND): BBa_K3102033 and 6-phosphogluconolactonase (PGL): BBa_K3102034 enzymes necessary for the oxidative pentose phosphate pathway.
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This "electron transfer improvement" composite is composed of Acetyl-CoA Synthetase (acs):BBa_K3102031 which is used to generate acetyl-coA by degrading acetate but also of G6P dehydrogenase (zwf):BBa_K3102032, 6-Phosphogluconate Dehydrogenase (gnd):BBa_K3102033 and 6-Phosphogluconolactonase (pgl): BBa_K3102034 enzymes necessary for the oxidative pentose phosphate pathway.
  
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The second one is composed of ABDEC Riboflavin’s operon: BBa_K3102029 and flavin biosynthesis gene (RibF): BBa_K3102030 to allow the extracellular production of the flavins, which are an electron transporter.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3102044 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3102044 parameters</partinfo>
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<!-- -->

Latest revision as of 13:48, 20 October 2019

Electron transfer improvement (ACS, ZWF, GND, PGL)

This "electron transfer improvement" composite is composed of Acetyl-CoA Synthetase (acs):BBa_K3102031 which is used to generate acetyl-coA by degrading acetate but also of G6P dehydrogenase (zwf):BBa_K3102032, 6-Phosphogluconate Dehydrogenase (gnd):BBa_K3102033 and 6-Phosphogluconolactonase (pgl): BBa_K3102034 enzymes necessary for the oxidative pentose phosphate pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2933
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2933
    Illegal NotI site found at 2814
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3490
    Illegal XhoI site found at 408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2933
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2933
    Illegal NgoMIV site found at 2206
    Illegal NgoMIV site found at 2923
    Illegal NgoMIV site found at 5493
    Illegal AgeI site found at 837
    Illegal AgeI site found at 4245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3975