Difference between revisions of "Part:BBa K2965035"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K2965035 short</partinfo> | ||
+ | This part is a crRNA of AsCas12a, it is complementary to HPV16 L1 sequence. There is a 5' TTTN PAM on the DNA strand opposite the target sequence. When AsCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K2965035 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K2965035 parameters</partinfo> |
Latest revision as of 09:59, 16 October 2019
As-crRNA-HPV16-2
This part is a crRNA of AsCas12a, it is complementary to HPV16 L1 sequence. There is a 5' TTTN PAM on the DNA strand opposite the target sequence. When AsCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]